234 £ Feiyuebio ELISA kit Customize Service

Feiyuebio Customize Service ELISA kit

Lead time

3-5 days

Specy

Customer"s sample

Range

According to customer"s requirements

Sensitivity

According to customer"s requirements

Notes

We can design and produce your required ELISA Kits for a wide range of targets and species. Just submit your detailed required information, Custom ELISA Kit service will be provided without any risk.

Our custom ELISA kits are able to reliably detect and quantify your unique antigens. Each kit of Feiyuebio is extensively tested, developed and validated to ensure its sensitivity to your target. Our high quality custom ELISA kits will play a great role in your test! Just provide specific antibodies, high purity antigen. Feiyuebio can develop specific ELISA Kit according to your personalized needs. These kits can be used for quantitative determination of protein concentration by ELISA sandwich method, competitive method (antigen assay), indirect method (antibody assay), or competitive method (small molecule compound assay).

The service is procedures include:

1, Project communication

Well know about the project background and expected goals to determine the development plan. Specific ELISA kits can be developed according to your individual needs. The content of the target substance can be quantitatively detected by ELISA sandwich method, competitive method (antigen determination), indirect method (antibody determination) or competitive method (small molecule compound determination) .

2, Immunogen preparation

a. Analyze the gene and amino acid sequence of the target protein, carry out antigenicity and expression analysis, codon optimization, and complete the whole gene synthesis.

b. Construct prokaryotic expression vector of recombinant protein.

c. Transform the E. Coli system, conduct recombinant protein expression induction and nickel column affinity purification to obtain 1-3 mg of purified recombinant protein with a purity of >85%.

d. Preparation of immunogens for animal immunization. For small target, molecular modification through structural analysis and coupling to carrier protein for immunization and detection.

3, Antibody design

Antibodie types need to prepare are determined according to the project communication. Generally, sandwich ELISA requires antibodies of different epitopes for pairing. It is recommended to prepare polyclonal antibodies and monoclonal antibodies at the same time. Ensure efficient pairing for the highest sensitivity. Other types of ELISA are selected according to detailed situation.

a, Experimental animal immune

Immunize 2 New Zealand rabbits and 5 BALB/c mice with the immunogen prepared in the first part for the preparation of polyclonal antibodies and monoclonal antibodies, respectively. Immunize 4-5 times, collect antiserum to detect titer, ELISA titer is more than 100,000, collect polyclonal antiserum and prepare monoclonal antibody hybridoma.

b, Monoclonal antibody hybridoma preparation

Select the cell fusion with the most immune -immune response to the recombinant protein and the natural protein, and screen the positive clone by ELISA to obtain a positive cell strain to obtain a positive cloning. Possess the positive cloning to the surface identification, reactive appraisal and affinity identification. Select different table positions, strong and highly affinity cells for subsequent antibody production.

c, Antibody production

The polyclonal antiserum was subjected to antigen affinity purification to obtain 10-20 mg of specific antibody.

Monoclonal Antibody Production-Select the desired positive cell line for cell expansion, prepare ascites, and obtain 3-5 mg of purified antibody by protein A affinity purification.

4, ELISA kit production

Why choose Feiyuebio

We focus developing research reagents and offer nearly 7,000 ELISA kits with nearly 20 years of experience since 2005.

Fully Risk-free Promise

Feiyuebio is ISO certificated, we provide reliable antibodies, proteins as well as ELISA kits. All our products are strictly validated with accurate QC standards to ensure product performance. If the product does not perform as promised in the protocol, please contact us with your detailed information before the expiry, we will provide professional technical support, replacement or even refund.

Notes:

Please store and use strictly according to the instructions in the protocol.

No mixing substitute reagents with other lots or sources.

No using the product after the expiry stated on the label.

For research use only, not for diagnostic or therapist use.